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antibodies against rip1  (ABclonal Biotechnology)

 
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    ABclonal Biotechnology antibodies against rip1
    Antibodies Against Rip1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against rip1/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    antibodies against rip1 - by Bioz Stars, 2026-02
    90/100 stars

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    Fig. 6 Effects of necrosta- tin-1 s on <t>RIP1/3</t> activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group
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    Fig. 6 Effects of necrosta- tin-1 s on <t>RIP1/3</t> activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group
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    Fig. 6 Effects of necrosta- tin-1 s on RIP1/3 activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group

    Journal: Molecular neurobiology

    Article Title: Electroacupuncture Inhibits Neuroinflammation Induced by Astrocytic Necroptosis Through RIP1/MLKL/TLR4 Pathway in a Mouse Model of Spinal Cord Injury.

    doi: 10.1007/s12035-023-03650-y

    Figure Lengend Snippet: Fig. 6 Effects of necrosta- tin-1 s on RIP1/3 activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group

    Article Snippet: Briefly, 300–500 μl tissue lysate was incubated with 0.5–2 μg antibody against RIP1 (sc133102; Santa Cruz; 1:1000), K63 (ab179434; Abcam; 1:1000), or GAPDH (ab181602; Abcam; 1:1000) for 3 h at 4 °C.

    Techniques: Activation Assay, Staining, Western Blot, Phospho-proteomics

    Fig. 6 Effects of necrosta- tin-1 s on RIP1/3 activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group

    Journal: Molecular neurobiology

    Article Title: Electroacupuncture Inhibits Neuroinflammation Induced by Astrocytic Necroptosis Through RIP1/MLKL/TLR4 Pathway in a Mouse Model of Spinal Cord Injury.

    doi: 10.1007/s12035-023-03650-y

    Figure Lengend Snippet: Fig. 6 Effects of necrosta- tin-1 s on RIP1/3 activation. a Representative immunofluo- rescence staining of GFAP/P- MLKL (scale bar = 20 μm). b, c, d, e Western blot analysis of RIP1, RIP3, and P-MLKL. EA, electroacupuncture; SCI, spinal cord injury; Nec-1 + EA, necrostatin-1 s combine with EA treatment. DAPI, 4′6-diami- dino-2-phenylindole; GFAP, glial fibrillary acidic protein; MLKL, mixed-spectrum kinase structural domain-like proteins; P-MLKL, phosphorylation of MLKL; RIP1/3, receptor- interacting protein kinase 1/3. All data are mean ± SD (n = 6/ group). *** P < 0.001 vs. sham group; ### P < 0.001 vs. SCI group; ^^^ P < 0.001 vs. Nec-1 group; & P < 0.001 vs. EA group

    Article Snippet: Samples were subjected to 12% SDS-PAGE gel electrophoresis, transferred to a PVDF membrane, and incubated with a primary antibody against RIP1 (17,519–1-AP; Proteintech; 1:1000), RIP3 (17,563–1-AP; Proteintech; 1:1000), MLKL (21,066–1-AP; Proteintech; 1:1000), P-MLKL (ab196436; Abcam; 1:1000), HMGB1 (10,829–1-AP; Proteintech; 1:1000), TLR4 (19,811–1-AP; Proteintech; 1:1000), MyD88 (bs-1047R; Bioss; 1:1000), β-actin (bs-1047R; Bioss; 1:1000) antibodies, or GAPDH (ab181602; Abcam; 1:1000) overnight at 4°C and then with an goat-anti-rabbit IgG H&L/HRP antibody (bs0295G-HRP; Bioss; 1:10,000) for 1 h. The bands were visualized using a chemiluminescence method, captured by IMAGE STUDIO imaging system, and analyzed by ImageJ.

    Techniques: Activation Assay, Staining, Western Blot, Phospho-proteomics